SGK, active, truncated NT 60aa, Recombinant, Human, GST-Tag (SGK1, Serum and Glucocorticoid-regulated Protein Kinase) discontinued. see S1010-85E

Dry Ice

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Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family. SGK1 is activated in vitro by 3-phosphoinositide- dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1, mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1. While both kinases can phosphorylate Thr-32, SGK1 displays a marked preference for Ser-315 whereas Akt favors Ser-253. These findings suggest that SGK1 and Akt may coordinately regulate the function of FKHRL1 by phosphorylating this transcription factor at distinct sites. Like PKB, SGK1 preferentially phosphorylates Ser and Thr residues that lie in Arg-Xaa-Arg-Xaa-Xaa- Ser/Thr motifs. The SGK1 gene has recently been identified as an important aldosterone-induced protein kinase that mediates trafficking of the renal epithelial Na(+) channel (ENaC) to the cell membrane. Thus, SGK1 is an appealing candidate for blood pressure regulation and possibly essential hypertension (3).

Recombinant protein corresponding to active human SGK-1 (truncated NT 60aa) produced in Sf9 insect cells, fused to GST-Tag at N-terminal, . Produced in a baculovirus expression system.

Specific Activity:
~60nmol/min/mg

Activity Assay:
Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30°C using a final concentration of 50uM [32P] ATP.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.