SGK1, aa399-412 (Serum and Glucocorticoid-regulated Protein Kinase 1)

Molecular Biology / MB-Protein Kinases

-20°C

Supplied as a white to off-white lyophilized powder.

Synthetic peptide (CGKSPDSVLVTASVK) (linear) corresponding to aa399-412 of human SGK1.

Serum and glucocorticoid-inducible kinases (SGKs) belong to a family of serine/threonine kinases that are regulated at both the transcriptional and posttranslational levels by external stimuli. SGKs are members of the AGC subfamily that includes the PKC isoforms, cyclic-AMP-dependent PKA and p90RSK. There are three isoforms, SGK1, SGK2 and SGK3. The transcriptional regulation of the two closely related isoforms, SGK2 and SGK3, are not well known. They can be activated by phosphorylation. SGK3 has been implicated in the IL-3 mediated survival of hematopoietic cells. SGK1 contains a catalytic domain that is ~45-55% homologous to the catalytic domains of PKA, PKB, PKC-zeta and rat p70S6K/p85 S6K kinases which propagate cell signalling cascades associated with the Positive Control of cell growth, differentiation and cell survival. The availability and function of SGK1 is regulated at three distinct levels of cellular control. First, SGK1 gene expression is strongly stimulated by hormonal and nonhormonal cues. Second, like PKB, SGK1 is phosphorylated and enzymatically activated as a downstream component of the PI 3-kinase signalling cascade that mediates the mitogenic and cell survival responses to many growth factors and insulin. Finally, the subcellular localization of SGK1 is controlled by the cell cycle and exposure to specific hormones and environmental stress stimuli. SGK1 plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion (1-4).

Applications:
Suitable for use in Western Blot and Inhibition assay when used with anti-SGK1. Other applications not tested.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.