Smad Ubiquitination Regulatory Factor-1,N-terminal FLAG-Tag,Recombinant,Human (Smad Ubiquitination Regulatory Factor 1,Smurf1,E3 Ubiquitin Ligase SMURF1,HGNC:16807,KIAA1625,Smad-specific E3 Ubiquitin Ligase 1,SMAD-specif

Dry Ice

70

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 100ug/ml FLAG peptide, 3mM DTT, 20% glycerol.

Source:
Recombinant corresponding to aa150-end of human SMURF 1, fused with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_020429).

Specific Activity:
0.0055pmol/min/ug Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotin-Ubiquitin (0.5ug)and Smurf1 (0-1ug) in 50mM Tris pH 7.4)
incubate for 30min at 37ºC, then add to streptavidin coated plates and incubate for
30min at 37ºC. Add antibody against Smurf1, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (lambda-exc=330 nm, lambda-em=620 nm).

Applications:
Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening
inhibitors and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 1, Smurf1, E3 Ubiquitin Ligase SMURF1, HGNC:16807, KIAA1625, Smad-specific E3 Ubiquitin Ligase 1, SMAD-specific E3 Ubiquitin Prote