SNRPF, aa1-86, Recombinant, Human (Small Nuclear Ribonucleoprotein Polypeptide F, snRNP-F, Sm Protein F, SmF, Sm-F, PBSCF)

Blue Ice

11, 8

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 1mM EDTA, 10% glycerol.

Small nuclear ribonucleoprotein polypeptide F, also known SNRPF, belongs to the snRNP Sm proteins family. There are at least seven isoforms, E, F, G, D1, D2, D3 and B/B. This class of common proteins plays an essential role in the biogenesis of the snRNPs. In addition, these proteins represent the major targets for the so-called anti-Sm auto-antibodies which are diagnostic for systemic lupus erythematosus (SLE). SNRPF aid in the cytoplasmic construction of the UsnRNPs by binding to aconserved Sm site on UsnRNA and forming a stable snRNP core complex.

Source:
Recombinant corresponding to human SNRPF, fused to His-tag at N-terminus, expressed in E. coli.

Molecular Weight:
~11.8kD

Sequences of Amino Acids:
MGSSHHHHHH SSGLVPRGSH MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.