Tissue, Membrane Protein, Human Adult Normal, Epididymis HisTek™

Biologicals / Biologicals-Tissues-Membrane Protein, Human

-70°C

Supplied as a liquid in HEPES, pH 7.9, KCl, sucrose, sodium deoxycholate, NP-40.

Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Applications:
Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interaction, Tissue-Specific Expression Identification. Other applications not tested.

Recommended Dilutions:
Western Blot: 10-20ug/lane
Optimal dilutions to be determined by the researcher.

Quality Control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.