Tissue, Membrane Protein, Human Adult Normal, Heart, Atrium (right) HisTek™

Biologicals / Biologicals-Tissues-Membrane Protein, Human

-70°C

Supplied as a frozen liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.

Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Applications:
• Electrophoresis, Western Blotting and Immunoprecipitation
• Enzymatic Activity
• Protein-DNA Interaction
• Protein-Protein Interaction
• Tissue-Specific Expression Identification

Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.

Preparation:
Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70°C.

Quality Control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.

Storage and Stability:
For extended storage, keep frozen at -80°C in working aliquots. For continuous use, stored at 2-8°C. Repeated freezing and thawing is not recommended