Recombinant Human Ubiquitin-Conjugating Enzyme E2I , His tag

Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IB, and PML without the requirement of an E3 ligase.

Escherichia Coli.

Lyophilized from a 0.2m filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution UBE2I should be stored at 4C between 2-7 days and for future use below
-18C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.