Recombinant Human Cystatin C Active

Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S. Cystatin C, a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

HEK.

Greater than 95% as obsereved by SDS-PAGE.

The inhibitory function of Cystatin-C on papins protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5-20 ug/ml (0.3-1.5 uM) with a range of 1.56-50ug/ml Cystatin-C in presence of 0.55uM papain and 0.44uM L-BAPA. The activity is typically 0.3-1.5uM IC50.