Comparison

Recombinant Mouse Heat Shock Protein 27 European Partner

Item no. hsp-065-2ug
Manufacturer ProSpec
Amount 2ug
Category
Type Proteins
Specific against other
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products HSP65
Available
Synonyms
Protein Cpn60-2, groEL protein-2, 65 kDa antigen, Heat shock protein 65, Cell wall protein A, Antigen A, groL2, groEL-2
Introduction
Heat shock proteins induce pro-inflammatory cytokines. Mycobacterial HSPs participate in cytokine expression resulting from infection by M. tuberculosis. Furthermore, HSPs stabilize cellular proteins in response to various sources of stress or injury. HSP65 is one of the most essential defending immunogens against the tuberculosis infection. HSP65 is presented to human CD41 T cells in association with multiple HLA-DR molecules. The M. tuberculosis HSP65 signals through TLR4.
Description
Recombinant Mycobacterium Tuberculosis HSP65 is produced in E.coli and has a Mw of 57.4 kDa. The HSP65 protein is fused to His-Tag at N-Terminus and purified by stabdard chromatography techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The HSP65 protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na-phosphate pH-7.4, 130mM NaCl and 2.5mM KCl.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Amino acid sequence
HHHHHHGSAKTIAYDEEARRGLERGLNALADAVKV
TLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPY
EKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVRE
GLRNVAAGANPLGLKRGIEAVEKVTETLLKGAKEVET
KEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITV
EESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAV
LEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIEDVEGE
ALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAIL
TGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVE
GAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKL
AGGVAVKAGAATEVELKERKHRIEDAVRNAKAAVEEGIV
AGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPL
KQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLA
AGVADPVKVTRSALQNASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF.
Storage
Lyophilized HSP65 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution HSP65 should be stored at 4C between 2-7 days and for future use below
-18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 2ug
Available: In stock
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