Porcine Enteropeptidase/Enterokinase

Item no.	enz-267-100IU
Manufacturer	ProSpec
Amount	100IU
Category	Peptides & Proteins Enzymes
Type	Enzymes
Specific against	Porcine
ECLASS 10.1	32160410
ECLASS 11.0	32160410
UNSPSC	12352204
Similar products	pEnterokinase
Available
Synonyms	Enteropeptidase, EC 3 4 21 9, Enterokinase, Serine protease 7, ENTK, MGC133046
Introduction	Enteropeptidase or enterokinase is an enzymeinvolved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum's glands, the crypts of Lieberkhn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen(a zymogen) to trypsin, indirectly activating a number of pancreaticdigestive enzymes. Enteropeptidase is a serine proteaseenzyme(EC3.4.21.9). Enteropeptidase is a part of the Chymotrypsin-clan of serine proteases, and is structurally similar to these proteins.
Description	Porcine enteropeptidase is a specific protease which cleaves after the lysine at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave a site followed by proline. Theoretical Mw is 21, 880 Dalton, the apparent Mw on SDS-PAGE is about 40 kDa. If a fusion tag is located in the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. ProSpecs enterokinase is a highly purified enterokinase from porcine. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases.
Source	Porcine.
Physical Appearance	Sterile Liquid.
Formulation	2 IU/ul, 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Storage	One year when stored at -20C, one week at room temperature.

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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enz-267-100IU-safety-datasheet.pdf