Recombinant Rat Insulin Like Growth Factor-1

The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH, MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor, ' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2, MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986, Rosenfeld, 2003).

Escherichia Coli.

The protein was lyophilized with no additives.

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The ED50, was calculated 2 methods:
1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be 30ng/ml corresponding to a Specific Activity of 33, 334IU/mg.
2. Type 1 IGF receptor binding assay ED50 was found to be 10ng/ml corresponding to a Specific Activity of 100, 000IU/mg.