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Recombinant Bacillus subtilis D-alanine--D-alanyl carrier protein ligase (dltA)

Recombinant Bacillus subtilis D-alanine--D-alanyl carrier protein ligase (dltA)

Item no.	BM-RPC25629-1mg
Manufacturer	Biomatik
Amount	1mg
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	other
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	D-alanine--poly(phosphoribitol) ligase subunit 1 D-alanine-activating enzyme dae
Similar products	D-alanine--poly(phosphoribitol) ligase subunit 1 D-alanine-activating enzyme dae
Available
Gene Name	dltA
Alternative Names	D-alanine--poly(phosphoribitol) ligase subunit 1 D-alanine-activating enzyme dae
Uniprot	P39581
Source	E.coli
Expression Region	1-503aa
AA Sequence	MKLLHAIQTHAETYPQTDAFRSQGQSLTYQELWEQ SDRAAAAIQKRISGEKKSPILVYGHMEPHMIVSFL GSVKAGHPYIPVDLSIPSERIAKIIESSGAELLIH AAGLSIDAVGQQIQTVSAEELLENEGGSVSQDQWV KEHETFYIIYTSGSTGNPKGVQISAANLQSFTDWI CADFPVSGGKIFLNQAPFSFDLSVMDLYPCLQSGG TLHCVTKDAVNKPKVLFEELKKSGLNVWTSTPSFV QMCLMDPGFSQDLLPHADTFMFCGEVLPVSVAKAL LERFPKAKIFNTYGPTEATVAVTSVEITNDVISRS ESLPVGFAKPDMNIFIMDEEGQPLPEGEKGEIVIA GPSVSRGYLGEPELTEKAFFSHEGQWAYRTGDAGF IQDGQIFCQGRLDFQIKLHGYRMELEEIEFHVRQS QYVRSAVVIPYQPNGTVEYLIAAIVPEEHEFEKEF QLTSAIKKELAASLPAYMIPRKFIYQDHIQMTANG KIDRKRIGEEVLV
Sequence Info	Full Length
Tag Info	N-terminal 6xHis-tagged
Theoretical MW	59.8 kDa
Purity	>85% as determined by SDS-PAGE.
Storage Buffer	Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin Level	Not tested
Biological Activity	Not tested
Shipping Condition	Ice packs
Storage	Short term: -20CC; Long term: -80CC. Minimize freeze and thaw cycles.
Expiry Date	1 year
Restriction	For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Relevance	Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Function	Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Subcellular location	Cytoplasm
Protein Families	ATP-dependent AMP-binding enzyme family, DltA subfamily

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1mg

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Delivery expected until 10/2/2025

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