Recombinant Bovine viral diarrhea virus Genome polyprotein, partial

N/A

E.coli

MELITNELLYKTYKQKPVGVEEPVYDQAGNPLFGE RGAIHPQSTLKLPHKRGERNVPTSLASLPKRGDCR SGNSKGPVSGIYLKPGPLFYQDYKGPVYHRAPLEL FEEGSMCETTKRIGRVTGSDGKLYHIYICIDGCIT VKSATRSHQRVLRWVHNRLDCPLWVTSC

N-terminal 10xHis-tagged

>85% as determined by SDS-PAGE.

Not tested

Ice packs

1 year

Initial binding to target cell probably involves interaction of E with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis.P7 forms a leader sequence to properly orient NS2 in the membrane.Uncleaved NS2-3 is required for production of infectious virus.NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.NS4A is a cofactor for the NS3 protease activity.RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.