Recombinant Human Cartilage intermediate layer protein 1 (CILP), partial

Cartilage intermediate-layer protein

Baculovirus

EDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAY RSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWG RFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLA GEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDH EDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENL RACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDP MSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVR SRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAA CLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVN PMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNY GIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKS NVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAG TVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQ QPLIN

N-terminal MBP-tagged and C-terminal 6xHis-tagged

>85% as determined by SDS-PAGE.

Not tested

Ice packs

1 year

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.

Intervertebral disc disease (IDD)

Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in hip articular cartilage. Overexpressed in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease (CPPD). Expression in intervertebral disk tissue from individuals with lumbar disk disease increases as disk degeneration progresses.