Recombinant SARS-CoV-2 (2019-nCoV) NSP3 / Papain-like Protease (His Tag)

Stable for up to 1 year from date of receipt at -20°C to -80°C. It is recommended to store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Non-structural protein 3 is 1 of 15 chains cleaved from Replicase polyprotein 1ab. Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling. The coronaviral papain-like protease (PLpro) and 3C-like protease (3CLpro) are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.

Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-GlyAMC (RLRGGAMC)