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Recombinant Mouse SMAD2 Protein (His & GST Tag)

Recombinant Mouse SMAD2 Protein (His & GST Tag)

Item no.	ELS-PKSM040517-100ug
Manufacturer	Elabscience
Amount	100 ug
Quantity options	100 ug 20 ug
Category	Neuroscience Neural Lineage Markers Peptides & Proteins Recombinant Proteins Neurodegeneration Markers
Type	Proteins
Format	Lyophilized Powder
Specific against	Mouse (Murine, Mus musculus)
Host	Insect Cells, Baculovirus-Infected Insect Cells
Conjugate/Tag	HIS, GST
Purity	> 90 % as determined by reducing SDS-PAGE.
Sequence	Ser2-Ser467
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	7120426M23Rik, Madh2, Madr2, Smad-2, mMad2
Similar products	7120426M23Rik, Madh2, Madr2, mMad2, Smad-2
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Proteins / Others
Manufacturer - Conjugate / Tag	N-His-GST
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	80.0 kDa
Observed Molecular Weight	90 kDa
Background	SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 8.0, 10% glycerol, 3mM DTT Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Not validated for activity
Protein Construction	A DNA sequence encoding the mouse SMAD2 (Q62432-Isoform Long) (Ser2-Ser467) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ELS-PKSM040517-100ug-datasheet.pdf

ELS-PKSM040517-100ug-safety-datasheet.pdf