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Recombinant Human Polymerase II Polypeptide J3

Recombinant Human Polymerase II Polypeptide J3

Item no.	enz-654-2ug
Manufacturer	ProSpec
Amount	2ug
Category	Peptides & Proteins Enzymes
Type	Enzymes
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160410
ECLASS 11.0	32160410
UNSPSC	12352204
Similar products	POLR2J3
Available
Synonyms	Polymerase (RNA) II (DNA Directed) Polypeptide J3, DNA-Directed RNA Polymerase II Subunit 11, DNA-Directed RNA Polymerase II Subunit RPB11-B2, DNA-Directed RNA Polymerase II Subunit J3, RNA Polymerase II Subunit B11-B2, POLR2J2, RPB11b2, RPB11b1
Introduction	DNA-directed RNA polymerase II subunit RPB11-b2 (POLR2J3) is a member of the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exists in 3 variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Description	POLR2J3 Human Recombinant produced in E. coli is a single polypeptide chain containing 138 amino acids (1-115) and having a molecular mass of 15.5 kDa. POLR2J3 is fused to a 23 amino acid His-tag at N-terminus purified by proprietary chromatographic techniques.
Source	E.coli.
Physical Appearance	Sterile Filtered colorless solution.
Formulation	The POLR2J3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 40% glycerol.
Purity	Greater than 95% as determined by SDS-PAGE.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Amino acid sequence	MGSSHHHHHH SSGLVPRGSH MGS MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
Storage	Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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enz-654-2ug-safety-datasheet.pdf