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MANF, human recombinant

MANF, human recombinant

Item no.	ICO-P-101-100-100ug
Manufacturer	Icosagen
Amount	100ug
Category	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	Human (Homo sapiens)
Host	Mammalian cell
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Mesencephalic astrocyte-derived neurotrophic factor – MANF; ARMET; ARP
Available
Background	MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
Bioproperties	Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke
Buffer	PBS pH 7.4, concentration
Concentration	1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
Endotoxine	Less than 1EU/mg of protein as determined by LAL method
Molecular Weight	Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids
Purification	Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media
References	Lindahl, M et al. 2014. MANF is Indispensable for the Proliferation and Survival of Pancreatic beta Cells. Cell Reports 7, 366-375 Bäck, S et al. 2013. Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson's disease.Brain and Behavior 3(2): 75-88
Storage	Store at -70C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Uniprot ID	P55145

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ug

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Delivery expected until 9/25/2025

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