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Recombinant SARS-CoV Nucleoprotein / NP Protein (His Tag)

Recombinant SARS-CoV Nucleoprotein / NP Protein (His Tag)

Item no.	PKSV030248-100
Manufacturer	Elabscience
Amount	100 ug
Category	Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized Powder
Specific against	Coronavirus
Host	Insect Cells, Baculovirus-Infected Insect Cells
Conjugate/Tag	HIS
Purity	> 80 % as determined by reducing SDS-PAGE.
Sequence	Met1-Ala422
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	SARS, coronavirus NP Protein, coronavirus Nucleocapsid Protein, coronavirus Nucleoprotein Protein, cov np Protein, ncov NP Protein, novel coronavirus NP Protein, novel coronavirus Nucleocapsid Protein, novel coronavirus Nucleoprotein
Shipping Condition	Cool pack
Available
Specificity	SARS
Manufacturer - Category	Recombinant Proteins / Viral Proteins
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	47.5 kDa
Observed Molecular Weight	47.1 kDa
Background	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Not validated for activity

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

PKSV030248-100-datasheet.pdf

PKSV030248-100-safety-datasheet.pdf