IL-2, N-terminal ProX((R)) tag, C-terminal His(6) tag

-70°C

Human

IL-2, rec., N-terminal ProX##(R)### tag, C-terminal His$$6$$$ tag

[Background]
IL-2 (Accession No. P60568), pin-point labeled with TAMRA, was produced in the E. coli cell-free translation system in the presence of TAMRA-C6-p-aminophenylalanine (TAMRA-aminophenylalanine) amber suppressor tRNA, which is the one ofthe CloverDirect##TM### tRNA reagents for site-directed protein labeling 1). Using amber suppression 2), TAMRA-aminophenylalanine is site-specifically incorporated at the amber stop codon (UAG) position. TAMRA-IL-2 has the N-terminal ProX##(R)### tag, which is the sequence tag optimized for high productivity and high introducing efficiency of TAMRA-aminophenylalanine, and has the C-terminal hexahistidine tag. The concentration of Pin-point TAMRA-