TransScript® IV Reverse Transcriptase [M-MLV, RNase H-] (Anti Inhibition High Temperature RT)

RT-PCR

TransStart® IV Reverse Transcriptase is an M-MLV reverse transcriptase with high thermostability expressed and purified from E. coli after genetic modification. It enables a board range reaction temperature (42°C-65°C) and good resistance to inhibitors, with ultra-high thermal stability and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure when synthesizing the first strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of fast reaction speed, high sensitivity, high specificity, high yield (more full-length cDNA), and long half-life.


Features
Wide range of applications: severely degraded RNA can be used as a template for reverse transcription.
Agnostic to inhibitors remaining in RNA.
Fast reaction speed: only 10 minutes for reverse transcription.
Strong synthesis ability: synthesis length up to 20kb.
High thermal stability: reaction temperature 42°C-65°C.
High sensitivity: high detection rate for trace RNA.


Applications
High-copy number and low-copy number gene detection.
High GC or complex secondary structure RNA template.
For difficult-to-handle RNA sample: degraded RNA template and RNA template containing RT enzyme inhibitor.
cDNA library construction, primer extension, 3’ and 5’ RACE.


Storage
at -20 ℃ for two years


Shipping
Dry ice (-70 ℃)