Proteinase K

Proteinase K is a nonspecific serine protease that will hydrolyze a variety of peptide bands. Proteinase K is active in a broad
range of temperature and buffers. It cannot be inactivated by metal ions, chelating agents (e.g., EDTA), or detergents such as
SDS.


• Active in a wide range of buffers and pH value.
• Incubation temperature: 55-65℃; optimal temperature: 58℃.
• Incubation time: 15 minutes to 48 hours; optimal incubation time: 2 hours.


Applications
• Preparation of DNA and RNA.
• Inactivation of RNase, DNase and enzymes.
• Isolation of genomic DNA.
• Isolation of RNA.


Storage
At room temperature (15℃-25℃) for one year; at -20℃ for two years.



Shipping
At room temperature