Protease Inhibitor Cocktail, Set X, EDTA-Free

Molecular Biology / MB-Protease Inhibitors

-20°C

Supplied as a lyophilized powder. Reconstitute with 1ml dH2O.

A cocktail of five protease inhibitors for the inhibition of a broad range of proteases, but not metalloproteases. This cocktail is recommended for use with bacterial cells, animal tissues and plant extracts. Each vial, when reconstituted with 1ml H2O, yields a 100X stock solution. When diluted to 1X the cocktail contains 100uM AEBSF, HCl, 1uM Leupeptin Hemisulfate, 400uM Benzamidine, HCl, 1uM E-64 and 150nM Aprotinin. Available as as a set of 5x1ml.

Primary Target:
Proteases

Solubility:
H2O

Kit Components:
AEBSF, HCl: 1X100uM
Leupeptin Hemisulfate: 1X1uM
Benzamidine, HCl: 1X400uM
E-64: 1X1uM
Aprotinin: 1X150nM

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.