Bionanocapsule-ZZ (BNC-ZZ)

For cell specific transfection of DNA, and detection probe for antigen-antibody complexThe BNC-ZZ is a type of bionanocapsule (hepatitis B virus surface antigen expressing hollow nano-particle) that can easily bind with desired antibodies by simple mixing, and developed for the purposes that 1) recognition and delivery of desired cells using the specificity of antibody against cell surface antigen such as receptors, and 2) easy-to-use detection tool of antigen-antibody complexes.

Basic type of BNC (BNC-L) is composed of L-protein of hepatitis B virus surface antigen which is floating on the particle made from lipid bi-layer. The BNC itself has ability to deliver its content into cytoplasm. Among three regions of L-protein, BNC-ZZ expresses zz-tag amino acids sequence in Pre-S1 region, the outmost region. Because zz-tag binds Fc region of antibodies, BNC-ZZ can binds to antibodies without affecting the antigen recognition site.

Thus, BNC-ZZ can be used for, depending on the antibody used, cell-specific DNA delivery in vitro, detection of antigen-antibody complex by using pre-labeled BNC-ZZ, and other applications. Application: Cell specific transfection for DNA and others

Detection probe for antigen-antibody complex. For example, as a replacement of the secondary antibody in various systems, such as western blotting and ELISA.