Cytoplasmic Dynein Inhibitor, Ciliobrevin D (2-(7-chloro-4-oxo-3,4-dihydroquinazolin-2(1H)-ylidene)-3-(2,4-dichlorophenyl)-3-oxopropanenitrile)(AAA+ ATPase Motor Cytoplasmic Dynein Inhibitor)

Blue Ice

392, 6

Supplied as an orange powder.

A cell-permeable benzoyl dihydroquinazolinone derivative that acts as a reversible and specific blocker of AAA+ ATPase motor cytoplasmic dynein and disrupts spindle pole focusing, cold-stable microtubule formation, kinetochore-microtubule attachment, and melanosome aggregation in a dose-dependent manner (~10-40uM). Shown to perturb protein trafficking within the primary cilia and cause a significant increase in intraflagellar transport protein 88 (IFT88) levels at the distal tip of primary cilia. Reported to disrupt primary cilium-dependent Gli regulation and block Hedgehog signaling.

Synonyms:
2-(7-chloro-4-oxo-3, 4-dihydroquinazolin-2(1H)-ylidene)-3-(2, 4-dichlorophenyl)-3-oxopropanenitrile, AAA+ ATPase motor cytoplasmic dynein inhibitor

CAS No:
1370554-01-0

Molecular Formula:
C₁₇H₈Cl₃N₃O₂

Molecular Weight:
392.6

Solubility:
DMSO (2.5mg/ml)

Form:
Supplied as an orange powder.

Purity:
≥99% (HPLC)

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.