Mitochondrial Fusion Promoter, M1 ((E)-4-Chloro-2-(1-(2-(2,4,6-trichlorophenyl)hydrazono)ethyl)phenol)

Blue Ice

364, 1

Supplied as an off-white solid.

A cell-permeable phenylhydrazone that restores mitochondrial tubular network formation in MEF lacking either of the two outer mitochondrial membrane (OMM) mitofusins (EC50=5.3 and 4.42 mM, respectively, in Mfn1 or Mfn2 knockout MEF cells) or in MPP+-treated SH-SY5Y cells (5uM 24h), but not in MEF lacking both Mfn1/2 or the inner mitochondrial membrane (IMM) fusion mediator Opa1 (optic atrophy1). M1 (5uM 24h) is reported to boost the downregulated ATP5A & ATP5B protein level in Mfn1 or Mfn2 knockout MEF to the wild-type MEF level and ATPase inhibitor oligomycin at 5uM is shown to completely offset the mitochondrial fusion effect by 5uM M1 in Mfn1 knockout MEF. Comparing to mdivi-1, M1 exerts its effect via promoting fusion rather than inhibiting fission or division.

CAS Number:
219315-22-7

Formula:
C14H10Cl4N2O

Molecular Weight:
364.1

Solubility:
DMSO (25mg/ml)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.