Protein A (Sepharose)

Molecular Biology / MB-BioSep Protein A/G/L

4°C Do Not Freeze

Supplied as a 50% suspension (5 ml Protein A-Sepharose® 4B + 5 ml PBS) in 10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin

Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants, and other biological fluids. These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments. Because Protein A and Protein G vary widely in their abilities to bind individual immunoglobulins, consult the table below before proceeding with your application. It should be noted that the Fc binding characteristics of naturally produced Protein A and Protein G are identical to their recombinant counterparts.

Protein-A/Sepharose® ratio: ~2.7mg Protein A per ml beads
Binding capacity: ~22mg Rabbit IgG per ml beads
Protein A Molecular Weight: 42kD (native)
Protein A Ig Binding Sites: 4 per molecule
Albumin Binding Sites/molecule: none

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C. Freezing will damage or destroy its performance.