Protein G (HRP)

Molecular Biology / MB-BioSep Protein A/G/L

-20°C

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA. Labeled with horseradish peroxidase (HRP).

Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:10, 000-1:40, 000 is recommended for blotting rabbit IgG.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.