(-)-Blebbistatin

Description: (-)-Blebbistatin is a potent, selective, reversible and cell-permeable small molecule inhibitor for non muscle myosin II ATPase with IC50 of around 2 uM in cell-free assays. It has been extensively used in research community to inhibit heart muscle myosin, non-muscle myosin II, and skeletal muscle myosin. As a cell-permeable molecule with high affinity and selectivity toward the myosin II contractile molecule, (-)-Blebbistatin preferentially binds to the myosin-ADP-Pi complex to slow down phosphate release. The inhibitor completely eliminate contraction of activity of actin-activated Mg-ATPase and motility of myosins II for several species in vitro (IC50 = 0.5-5.0 uM), but it has poor effects on smooth muscle myosin II (IC50 =80 uM) and myosins I, V, and X.

References: Science. 2003 Mar 14; 299(5613):1743-7; J Muscle Res Cell Motil. 2004; 25(4-5):337-41.

Synonym: (S)-Blebbistatin; Blebbistatin.

Chemical Name: 1, 2, 3, 3a-tetrahydro-3aS-hydroxy-6-methyl-1-phenyl-4H-Pyrrolo[2, 3-b]quinolin-4-one

InChi Key: LZAXPYOBKSJSEX-GOSISDBHSA-N

InChi Code: InChI=1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8, 11, 22H, 9-10H2, 1H3/t18-/m1/s1

SMILES Code: O=C1[C]2(O)C(N(C3=CC=CC=C3)CC2)=NC4=C1C=C(C)C=C4