Calcifediol (25-hydroxy Vitamin D3)

Description: Calcifediol (also known as 25-hydroxyvitamin D3; 25-hydroxy VD3) is the major circulating metabolite of vitamin D3 in the blood and is the form that is tested in medicine to determine vitamin D deficiency, namely, the test of 25-hydroxy vitamin D (calcifediol) levels to evaluate how much vitamin D is in the body. Calcifediol acts as a competitive inhibitor with an apparent Ki of 3.9 uM. It also suppresses PTH secretion and mRNA (ED50=2 nM). Calcifediol induced CYP24A1 expression with EC50 at 70 nM. Calcifediol stimulated the expression of thrombomodulin with EC50 at 10-100 nM. Confocal microscopy revealed that calcifediol at 0.1 - 10 uM induced VDR translocation into the nucleus dose-dependently; the VDR localization pattern was similar in cells treated with calcitriol.

References: Cardiovasc Drugs Ther. 2011 Jun; 25(3):215-22; Hypertension. 1994 Aug; 24(2):176-82.

Related CAS#: 19356-17-3; 63283-36-3 (Calcifediol); 63283-36-3 (Calcifediol monohydrate); 127516-23-8 (Impurity of Doxercalciferol); 78782-98-6 Calcifediol-D6, the deuterated form of Calcifediol (25-hydroxy Vitamin D3); 67-96-9 (Dihydrotachysterol)