Incyclinide

Description: Incyclinide (formerly known as CMT-3 and COL-3; 6-demethyl, 6-deoxy, 4-dedimethylamino TC; a chemically modified tetracycline) is a matrix metalloproteinase (MMP) inhibitor, it acts by inducing extracellular matrix degradation, and inhibiting angiogenesis, tumor growth and invasion, and metastasis. CMT-3 has been shown to experimentally suppress prostate cancer, colon adenocarcinoma and melanoma invasiveness in cell culture and to inhibit tumor growth and metastasis in vivo and was used in the current in vitro study. Adding CMT-3 at final concentrations of 5--20microM inhibited MT1-MMP gelatinolytic and caseinolytic activity, blocked MT1-MMP activation of pro-MMP-2, and decreased invasiveness (using the Matrigel system) of HT-1080 fibrosarcoma cells. The inhibition of MT1-MMP by CMT-3 may partially explain the inhibition of cancer cell -mediated tissue breakdown and invasiveness by this non-antimicrobial tetracycline analog.

References: Arch Oral Biol. 2007 Jun; 52(6):571-8; Curr Med Chem. 2001 Feb; 8(3):257-60.