AP Magenta 3 Component Membrane Substrate

Ambient

Leinco’s AP Magenta 3 Component Membrane Substrate is a precipitating substrate used with the enzyme alkaline phosphatase designed for membrane blotting procedures. The TAC Buffer coupled with a substitute, naphthol, is enzymatically hydrolyzed. In the presence of hexazotized substrate, a fine magenta precipitate is produced at the sites of alkaline phosphatase activity.
Components:
1. TAC Buffer (Modified Gormori’s Tris Azo-Coupling Buffer)- 4 x 100 ml or 1 x 1 L
2. Initiator (Hexa-azonium salt initiator) - 1 x 8 ml or 1 x 20 ml
3. Magenta Solution - 1 x 8 ml or 1 x 20 ml