Loose Im7 4B Resin

The Im7 Resin contains a highly specific, resilient Im7 domain that binds the CL7 tag (~16 kDa) fused to target proteins. Following cleavage with the appropriate protease, the target protein releases from the Im7-bound CL7. Available in 1-, 2-, 5-, and 15-mL volumes of settled resin (50% suspension). Resin upholds its effectiveness for multiple reactivations. TriAltus’ Im7 4B Resin retains a 1:1 molar binding ratio during repeated uses. The Im7 4B Resin has been used to purify a variety of proteins – including membrane and multisubunit proteins. SIMPLICITY – In a single purification step, this protocol yields purified proteins that exhibit high yield, high purity, and a high activity level. REUSABILITY – The Im7 4Bresin is reusable with no observable loss in capacity. RESILIENCY – The Im7 4B Resin retains a 1:1 molar binding ratio after multiple reactivations in a variety of conditions, including various salt and detergent concentrations. BUFFER: 20 mM Tris-Cl pH 8.0, 0.5M NaCl, 5% glycerol, 0.05% sodium azide PARTICLE SIZE: Crosslinked agarose 4B beads (45 – 165 μM) PH STABILITY: The Im7 protein is stable on the beads at pH 3-10. However, CL7/Im7 binding is stable at pH 4.2 – 10 only.SALT STABILITY: ≤ 4M NaCl tested BINDING CAPACITY: ≥ 60 mg CL7/mL resin STORAGE/SHIPPING CONCENTRATION: 50/50 buffer/resin slurry. 1-, 2-, 5-, and 15-mL of settled resin in 2, 4, 10, and 30 mL of slurry, respectively. RECOMMENDED OPERATING TEMPERATURE:  4°C or room temperature REACTIVATION DETAILS:To remove the CL7 protein and reactivate the resin, wash the column with Gdn-HCl, exchanging it into physiological buffer.