IL-10

Item no.	RLT-100-049
Manufacturer	ReliaTech
Amount	10ug
Category	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Cytokines and Growth Factors
Format	Lyophilized
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Monkey (Cynomolgus, Simian), Bacteria (generic)
Host	E.coli
ECLASS 10.1	42030690
ECLASS 11.0	42030690
UNSPSC	12352202
Alias	IL10, CSIF, TGIF, GVHDS, IL-10, IL10A
Available
NCBI Gene ID	3586
Uniprot	P22301
Biological Activity	Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be = 2.0 ng/ml, corresponding to a specific activity of = 5 x 105 units/mg.
Buffer	5mM Sodium Phosphate, pH 7.2
Description	Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kDa protein that shares over 80% homology with the Epstein-Barr virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the TH2 cell response which results in elevated antibody production.
Endotoxin Levels	< 0.1 ng/µg of protein (<1EU/µg)
Length [aa]	161
Molecular Weight	18.6 kDa
mRNA RefSeq	NM_000572
Protein RefSeq	NP_000563.1
Protein Sequence	MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Purity Confirmation	> 98% by SDS-PAGE & HPLC analyses
Reconstitution	Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Stability And Storage	The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
Synonyms	IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Uniprot ID	P22301

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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