PDGF-AA

5154

The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).

NP_081182

NM_002607.5

NP_002598.5

> 95% by SDS-PAGE & visualized by silver stain

The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted PDGF-AA is best stored at -20 °C to -70 °C. Avoid repeated freeze-thaw cycles.

P04085