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5(6)-FAM, SE

5(6)-FAM, SE

Item no.	T01022
Manufacturer	Tenova
Amount	100mg
Category	Reagents & Chemicals Staining & Dyes Fluorescent Reagents
Type	Fluorescent dyes and probes
Specific against	other
ECLASS 10.1	30196090
ECLASS 11.0	30196090
UNSPSC	12000000
Alias	5 - (and - 6) - Carboxyfluorescein, succinimidyl ester
Available
Description	5(6)-FAM, SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate such as FITC.
Synonyms	5 - (and - 6) - Carboxyfluorescein, succinimidyl ester
CAS Number	117548-22-8
Molecular Weight	473.39
Molecular Formula	C25H15NO9
Excitation (nm)	494
Emission(nm)	519
Purity (HPLC)	&ge, 95%
Shipping Conditions	Room temperature
Solubility	DMSO, DMF
Storage	Store in freezer (-5 to -30°, C) and protect from light.
Regulatory Statement	For research use only.
References	1. Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700, 2. Sanders SJ (2000). Factor V Leiden genotyping using real-time fluorescent polymerase chain reaction. Mol Cell Probes 14, 249-53, 3. Brandis JW (1999). Dye structure affects Taq DNA polymerase terminator selectivity. Nucleic Acids Res 27, 1912-8.

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100mg

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