HIV-p24 ELISA Kit (One-step)

p24 is a highly conserved and most abundant major structural protein in HIV virions. It is a product encoded by the structural gene gag and plays a key role in the packaging and maturation of the virus. Lentiviral vector is a viral vector system modified on the basis of HIV-1 virus, which can efficiently transfer the target gene into cells. The content of p24 protein represents the number of lentivirus particles, therefore, the physical titer of lentivirus can be determined by assaying the p24 content by ELISA method.
This kit uses one-step Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to rapidly measure the amount of HIV-p24 in lentiviral supernatant. The entire incubation time is 70 minutes. The ELISA microplate in the kit is precoated with the HIV-p24 antibody. The virus lysate, standard/test sample, biotin-labeled HIV-p24 detection antibody and horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) are added to the microplate wells. After incubation, " coated antibody-HIV-p24 protein-detection antibody-Streptavidin-HRP" immune complex can be produced. After washing, the chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with intensity positively correlated with the concentration of HIV-p24 in the sample. The reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630
nm). A standard curve is drawn to calculate the concentration of HIV-p24 in the sample based on the absorbance value. This kit is highly specific, has high detection sensitivity and is convenient to use.


Storage
at 2~8°C in the dark for one year


Shipping
ice bag (4℃)