TAF1 TR-FRET, BioAssay™ Kit (BD1+BD2, Transcription Initiation Factor TFIID Subunit 1, Cell Cycle Gene 1 Protein)

Kits and Assays / Kits-Molecular Biology, BioAssay™

-20°C/-70°C

Kit comes in a convenient format with purified Europium-labeled TAF1 (BD1+BD2) protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.

The TAF1 (BD1+BD2) TR-FRET BioAssay™ Kit is designed to measure the inhibition of TAF1 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing trFluorTM europium (Eu)-labeled TAF1 (BD1+BD2), dye-labeled acceptor, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Applications:
Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.

Kit Components:
White, Nonbinding Corning, low volume, microtiter plate, 1x plate
TAF1 (BD1+BD2)-Eu, 1x3ug
BET Bromodomain Ligand, 1x50ul
Non-acetylated Ligand 1, 1x15ul
Dye-labeled acceptor, 1x20ul
3x BRD TR-FRET Assay Buffer 1, 1x4ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.