Tissue Factor (F3), BioAssay™ ELISA Kit (Mouse)

Kits and Assays / Kits-ELISA (Coagulation Factors), BioAssay™

4°C/-20°C

Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Sample Type:
Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and cell lysates

Intended Use:
For quantitative detection of mouse Tissue factor in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and cell lysates.

Sensitivity:
<10pg/ml

Range:
15.6-1000pg/ml

Specificity:
Natural and recombinant mouse Tissue factor

Test Principle:
This mouse Tissue factor BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Tissue factor has been precoated onto 96-well plates. Standards(NSO, A29-E251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tissue factor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Tissue factor amount of sample captured in plate.

Kit Components:
96-well plate precoated with mouse Tissue factor antibody, 1
Lyophilized recombinant mouse Tissue factor standard, 10ng/tube×2
Biotinylated mouse Tissue factor antibody, 130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)
Sample diluent buffer, 30ml
Antibody diluent buffer, 12ml
ABC diluent buffer, 12ml
TMB color developing agent, 10ml
TMB stop solution, 10ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.