Rt3 (Reverse Triiodothyronine) BioAssay™ ELISA Kit (Rat)

Kits and Assays / Kits-ELISA (Hormones & Steroids), BioAssay™

4°C/-20°C

For quantitative detection of Rt3 in rat serum, plasma, tissue homogenates and other biological fluids.

Detection Range:
93.75-6000pg/ml

Sensitivity:
56.25pg/ml

Precision:
Intra-Assay %CV: <8%
Inter-Assay %CV: <10%

Specificity:
This assay has high sensitivity and excellent specificity for detection of Rt3. No significant cross-reactivity or interference between Rt3 and analogues was observed.

Assay Principle:
This BioAssay™ kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit Components:
Microtiter Strips, 1x96 wells
Lyophilized Standard, 2x vial
Sample/Standard Dilution Buffer, 1x20ml
Antibody (Biotin) (Concentrated), 1x60ul
Antibody Dilution Buffer, 1x10ml
Streptavidin (HRP) (SABC), 1x120ul
SABC Dilution Buffer, 1x10ml
TMB Substrate, 1x10ml
Stop Solution, 1x10ml
Wash Buffer, 25X, 1x30ml

Storage and Stability:
Store unopened Microtiter Strips at 4ºC; store at -20ºC once opened. Store unopened Standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.