TLR7 (Toll-Like Receptor 7) BioAssay™ ELISA Kit (Rat)

Kits and Assays / Kits-ELISA (Pattern Recognition Receptors), BioAssay™

4°C/-20°C

For quantitative detection of rat TLR7 in serum, plasma, tissue homogenates and other biological fluids.

Detection Range:
0.313-20ng/ml

Sensitivity:
0.188ng/ml

Precision:
Intra-Assay %CV: <8%
Inter-Assay %CV: <10%

Specificity:
This assay has high sensitivity and excellent specificity for detection of TLR7. No significant cross-reactivity or interference between TLR7 and analogues was observed.

Assay Principle:
This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

Kit Components:
Microtiter Strips, 1x96 wells
Lyophilized Standard, 2x vial
Sample/Standard Dilution Buffer, 1x20ml
Antibody (Biotin) (Concentrated), 1x120ul
Antibody Dilution Buffer, 1x10ml
Streptavidin (HRP) (SABC), 1x120ul
SABC Dilution Buffer, 1x10ml
TMB Substrate, 1x10ml
Stop Solution, 1x10ml
Wash Buffer, 25X, 1x30ml

Storage and Stability:
Store unopened Microtiter Strips at 4ºC; store at -20ºC once opened. Store unopened Standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.