SPHK2, Recombinant, Human, full length (Sphingosine kinase 2, SK 2)

Dry Ice

69, 5

Supplied as a liquid in 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.

Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His-tag, MW=69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

MW 69.5kD, expressed in an Baculovirus infected Sf9 cell expression system.

Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D- erythro-sphingosine and [ -32P]ATP as substrates at pH 7.4 and 30 °C. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1pmol of S1P/min.

Assay buffer: 50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythro-sphingosine, and 1ug/ml sphingosine kinase 2.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.