Abl Kinase, Active, Recombinant, Human, His-Tag (ABL, Tyrosine-protein Kinase ABL1, Abelson Murine Leukemia Viral Oncogene Homolog 1, c-Abl, Proto-oncogene c-Abl, p150, ABL1, JTK7)

Dry Ice

33

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 20% glycerol, 3mM DTT.

The c-ABL proto-oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-ABL proto- oncogene undergoes a chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as a result of the fustion of c-ABL sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/ABL mRNA encoding activated ABL protein tyrosine kinase.

Source:
Recombinant corresponding to aa248-531 from human Abelson murine leukemia viral oncogene homolog 1, fused to His-tag at N-terminal, expressed in a Baculovirus-infected Sf9 cell expression system.

Molecular Weight:
~33kD

Applications:
Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Specific Activity:
~288pmol/min/ug

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.