Apolipoprotein E3, Recombinant, Human, His-Tag, T7-Tag

Blue Ice

26

Supplied as a liquid in 0.01M Tris, pH 7.2. No preservative added.

Sulfated glycoprotein-2 (SGP-2) is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood. It consists of two 40kD chains, alpha and beta, covalently joined by disulfide bonds. They established that SP-40, 40 is a member of the human complement system by directly demonstrating its presence within the S-protein-containing soluble variant of the C5b-9 complex, SC5b-9. SP-40, 40 is also called complement lysis inhibitor. It acts as a control mechanism of the complement cascade; specifically, it prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is the human analog of the rat protein present in high concentrations in the testis, sulfated glycoprotein-2. It is a 70kD protein associated with high-density lipoproteins (HDL) in human plasma. There is a single copy of the APOJ gene in the human and mouse genomes. The protein is synthesized as a 427aa polypeptide that is posttranslationally cleaved at an internal bond between arg205 and ser206. Two subunits, designated alpha (34-36kD), corresponding to residues 1-205, and beta (36-39kD), corresponding to residues 206-427, are associated through disulfide bonds. APOJ mRNA (1.9kb) in all but one tissue examined. Its concentration was relatively high in brain, ovary, testis, and liver, lower in heart, spleen, lung, and breast, and absent in T lymphocytes. Apolipoprotein J is distinct from other known apolipoproteins in molecular weight, subunit structure, and isoelectric point. ApoJ is induced in myocarditis and numerous other inflammatory injuries. Deficient and wildtype mice exhibited similar initial onset of myocarditis. Furthermore, autoantibodies against the primary antigen cardiac myosin were induced to the same extent. After resolution of inflammation, apoJ-deficient, but not wildtype, mice exhibited cardiac function impairment and severe myocardial scarring. These results suggested that apoJ normally limits progression of autoimmune myocarditis and protects the heart from postinflammatory tissue destruction.

Source:
Recombinant protein corresponding to human Apolipoprotein E3 with N-terminal fusion of T7-Tag (16aa) and C-terminal fusion of His-Tag (9aa), expressed in E. coli.

Molecular Weight:
~26kD

Applications:
Suitable for use in ELISA, Western Blot and Dot Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.