CASP3, Recombinant, Human, aa176-277, His-Tag (Caspase 3, CPP32, CPP32B, SCA-1)

Blue Ice

12

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.

CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Source:
Recombinant protein corresponding to aa176-277 from human CASP3, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~12kD (103aa)

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.