Angiotensin-Converting Enzyme 2, Recombinant, Mouse, aa18-740, His-Tag (ACE2)

Blue Ice

84, 5

Supplied as a liquid in PBS, pH 7.4, 10% glycerol.

ACE2, also known as angiotensin-converting enzyme 2, is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE2 cleaves angiotensin 1 to angiotensin 1-9 and angiotensin 2 to angiotensin 1-7 as a carboxypeptidase. It may be an important regulator of heart function and have a protective role in acute lung injury.

Source:
Recombinant protein corresponding to aa18-740 from mouse ACE2, fused to His-tag at C-terminal expressed in Insect cell.

Molecular Weight:
~84.5kD (731aa)

Amino Acid Sequence:
QSLTEENAKT FLNNFNQEAE DLSYQSSLAS STGKVCNPKN PQECLLLEPG LDEIMATSTD EHLHAYVRRK LMDTYPSYIS PTGCLPAHLL AWDLGHGDFR IKMCTKVTMD NFLTAHHEMG KWRWMVFRGE IPKEQWMKKW WEMKREIVGV KALENVVGAR NMDVKPLLNY FQPLFDWLKE RVSFYFFVTS PQNVSDVIPR SEVEDAIRMS WNYNTNITEE NAQKMSEAAA KWSAFYEEQS KTAQSFSLQE IQTPIIKRQL QALQQSGSSA LSADKNKQLN TILNTMSTIY YNSRLWAWEG WRAEVGKQLR PLYEEYVVLK NEMARANNYN DYGDYWRGDY EAEGADGYNY NRNQLIEDVE RTFAEIKPLY GDMWGRFWTN LYPLTVPFAQ KPNIDVTDAM MNQGWDAERI FQEAEKFFVS VGLPHMTQGF WANSMLTEPA DGRKVVCHPT HIQYDMAYAR QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLPSD FQEDSETEIN FLLKQALTIV GTLPFTYMLE VEPLPHDETY CDPASLFHVS NDYSFIRYYT RTIYQFQFQE ALCQAAKYNG SLHKCDISNS TEAGQKLLKM LSLGNSEPWT QNRNSFVGWN TEWSPYADQS IKVRISLKSA LGANAYEWTN NEMFLFRSSV AYAMRKYFSI IKNQTVPFLE EDVRVSDLKP RGRINDVFGL NDNSLEFLGI HPTLEPPYQPPVTLEHHHHH H

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.