Akt1, active, Recombinant, Human, GST-Tag (Rac PKa, PKBa)

Dry Ice

60, 2

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.

Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system.

Specific Activity:
~12pmol/min/ug

Assay Conditons:
Enzyme reaction is conducted in a buffer
containing 50mM HEPES, pH 7.5, 10mM
magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour.

Applications:
Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.