CD152 Human, IgG2a Mouse Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

Blue Ice

40, 1

Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, and 150mM sodium chloride, 0.5mg/ml Gentamicin Sulfate.

Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses. The CD152 Ig fusion protein has biological activity and binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2). CD152 Ig will block the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) monoclonal antibodies.

Functional Application: CD152 Ig binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2) (4, 5).  CD152 Ig blocks the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) mAbs.

Human CD152(CTLA-4) muIg Fusion Protein*(also binds to mouse CD80/CD86)

Molecular Structure: A soluble 110kD dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc

Transfectant Cell Line: BHK

Applications:
Suitable for use in ELISA, Flow Cytometry, and Antibody Blocking. Other applications not tested.

Recommended Dilution
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.