MAP Kinase p44/42,phosphorylated (Thr202,Tyr204),Immunohistochemistry Controls (MAPK3/MAPK1,Mitogen-activated Protein Kinase 1,MAP Kinase 1,MAPK 1,ERT1,Extracellular Signal-regulated Kinase 2,ERK-2,MAP Kinase Isoform p42

Molecular Biology / MB-Protein Kinases

4°C

p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY*, resulting in p38 activation.

Sections are cut freshly upon ordering.

Description:
Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western Blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment.

Applications:
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides.

Companion Products
• Phospho-p44/42 MAPK (Thr202/Tyr204) Rabbit mAb (IHC Preferred)
• M2352-31: Growth/Proliferation Marker: SignalStain® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Detection Kit
• Phospho-p44/42 MAPK (Thr202/Tyr204) Blocking Peptide (IHC Specific)
• p44/42 MAP Kinase Rabbit mAb
• p44/42 MAP Kinase Blocking Peptide
• p44/42 MAP Kinase (L34F12) Mouse mAb
• p44/42 MAP Kinase Blocking Peptide
• M2360-02: p44/42 MAP Kinase Antibody
• M2352-11A: p42 MAP Kinase (Erk2) Antibody

Storage:
Store at 4°C. Optimal staining is achieved if slides are stained following our standard IHC protocols and are used within 8 weeks of assay date; however, signals may persist beyond two months.

MAP Kinase p44/42, phosphorylated (Thr202, Tyr204), Immunohistochemistry Controls (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-