Myelin Basic Protein, Human Brain, Lyophilized (MBP)

Molecular Biology / MB-Myelin

-20°C

Supplied as a lyophilized powder. No preservatives added. Reconstitute with 10mM HCl.

In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.

Purity:
≥95% (SDS-PAGE)

Form:
Supplied as a lyophilized powder. No preservatives added. Reconstitute with 10mM HCl.

Applications:
Suitable for use in ELISA or for antigen applications in immunological protocols. Immunoreactivity is verified using polyclonal and monoclonal antibodies against MBP. Other applications not tested..

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Molecular Weight:
MBP contains 14.2kD (13.0 minor) and 18.5kD (17.2 minor) isoforms as well as their dimers (13.5% SDS-PAGE, non-reducing conditions).

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with 10mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Blood sample from the tissue donor has been tested negative for antibodies against HIV-2, HIV-2 and HCV; it has also been tested negative for HBsAg.  As no test can guarantee a product to be non-infectious, all material derived from human fluids or tissues should be considered as potentially infectious.